other:Article Title: LAMC1 Aggravates Diabetic Retinopathy Through PI3K/AKT Signaling-Regulated Epithelial-Mesenchymal Transition in Retinal Pigment Epithelial Cells
Article Snippet: LAMC1 Cell Signaling Technology (92921) 1:1000 E-cadherin ABclonal (A20798) 1:1000 N-cadherin Proteintech (22018-1-AP) 1:2000 Vimentin Abcam (ab92547) 1:1000 Snail ABclonal (A11794) 1:1000 PI3K Cell Signaling Technology (4292) 1:1000 p-PI3K Affinity Biosciences (AF3242) 1:1000 Jo ur na l P re -p ro of AKT Cell Signaling Technology (9272) 1:1000 p-AKT Cell Signaling Technology (9271) 1:1000 GAPDH ABclonal (A19056) 1:10000 Goat Anti-Rabbit IgG H&L (HRP) ABclonal (AS014) 1:10000
Article Title: Indirubin attenuates sepsis by targeting the EGFR/SRC/PI3K and NF-κB/MAPK signaling pathways in macrophages
Article Snippet: Antibodies Suppliers (catalog number) Antibodies Suppliers (catalog number) EGFR Cell Signaling Technology (2232) p-EGFR Cell Signaling Technology (2234) Src Cell Signaling Technology (2108) p-Src Cell Signaling Technology (2101) PI3K Cell Signaling Technology (4292) p-PI3K Cell Signaling Technology (4228) Akt Cell Signaling Technology (9272) p-Akt Cell Signaling Technology (9271) IκBα Cell Signaling Technology (4812) p-IκBα Cell Signaling Technology (2859) p65 Cell Signaling Technology (8242) p-p65 Cell Signaling Technology (3033) ERK Cell Signaling Technology (4695) p-ERK Cell Signaling Technology (4370) JNK Wanlei Biotechnology (WL01295) p-JNK Wanlei Biotechnology (WL01813) p38 Wanlei Biotechnology (WL00764) p-p38 Wanlei Biotechnology (WL03428) GAPDH Cell Signaling Technology (2118) β-actin Cell Signaling Technology (4970) Goat anti-rabbit IgG-HRP Proteintech (SA00001-2) Goat anti-mouse IgG-HRP Proteintech (SA00001-1)
Article Title: Indirubin attenuates sepsis by targeting the EGFR/SRC/PI3K and NF-κB/MAPK signaling pathways in macrophages
Article Snippet: Antibodies Suppliers (catalog number) Antibodies Suppliers (catalog number) EGFR Cell Signaling Technology (2232) p-EGFR Cell Signaling Technology (2234) Src Cell Signaling Technology (2108) p-Src Cell Signaling Technology (2101) PI3K Cell Signaling Technology (4292) p-PI3K Cell Signaling Technology (4228) Akt Cell Signaling Technology (9272) p-Akt Cell Signaling Technology (9271) IκBα Cell Signaling Technology (4812) p-IκBα Cell Signaling Technology (2859) p65 Cell Signaling Technology (8242) p-p65 Cell Signaling Technology (3033) ERK Cell Signaling Technology (4695) p-ERK Cell Signaling Technology (4370) JNK Wanlei Biotechnology (WL01295) p-JNK Wanlei Biotechnology (WL01813) p38 Wanlei Biotechnology (WL00764) p-p38 Wanlei Biotechnology (WL03428) GAPDH Cell Signaling Technology (2118) β-actin Cell Signaling Technology (4970) Goat anti-rabbit IgG-HRP Proteintech (SA00001-2) Goat anti-mouse IgG-HRP Proteintech (SA00001-1) Table S2.
Article Title: LAMC1 Aggravates Diabetic Retinopathy Through PI3K/AKT Signaling-Regulated Epithelial-Mesenchymal Transition in Retinal Pigment Epithelial Cells
Article Snippet: LAMC1 Cell Signaling Technology (92921) 1:1000 E-cadherin ABclonal (A20798) 1:1000 N-cadherin Proteintech (22018-1-AP) 1:2000 Vimentin Abcam (ab92547) 1:1000 Snail ABclonal (A11794) 1:1000 PI3K Cell Signaling Technology (4292) 1:1000 p-PI3K Affinity Biosciences (AF3242) 1:1000 AKT Cell Signaling Technology (9272) 1:1000 p-AKT Cell Signaling Technology (9271) 1:1000 GAPDH ABclonal (A19056) 1:10000 Goat Anti-Rabbit IgG H&L (HRP) ABclonal (AS014) 1:10000 Jo ur na l P re -p ro of
Western Blot:Article Title: PACAP–Sirtuin3 alleviates cognitive impairment through autophagy in Alzheimer’s disease
Article Snippet: .. ATG5 Forward GCTTTTGCCAAGAGTCAGCTAT Reverse AACCAATTGGATAATGCCATTTCAG Beclin1 Forward AGGAACTCACAGCTCCATTACT Reverse CTCTCCTGAGTTAGCCTCTTCC GAPDH Forward GACCCCTTCATTGACCTCAAC Reverse TTCTCCATGGTGGTGAAGA Supplementary Table S2 Antibodies used in western blot Target Supplier Catalog Number Concentration Aβ1-16 Biolegend 803001 1: 1000 Tau-5 BD Biosciences 556319 1: 1000 PACAP Invitrogen ZP006 1: 1000 Map1LC3 Cell Signaling Technology 4108s 1: 1000 Atg5 Cell Signaling Technology 2630s 1: 1000 Beclin1 Cell Signaling Technology 3738s 1: 1000 Sirt3 Cell Signaling Technology 5490s 1: 1000 PI3K Cell Signaling Technology 4249s 1: 1000 P-PI3K Cell Signaling Technology 4228s 1: 1000 AKT Cell Signaling Technology 9272s 1: 1000 P-AKT Cell Signaling Technology 4060s 1: 1000 mTOR Cell Signaling Technology 2983s 1: 1000 p-mTOR Cell Signaling Technology 2971s 1: 1000 AMPK Cell Signaling Technology 2793s 1: 1000 p-AMPK Cell Signaling Technology 2535s 1: 1000 Beta Actin Santa Cruz Biotechnology sc-47778 1: 5000 Goat Anti-Rabbit IgG ComWin Biotech CW0103S 1: 5000 Goat Anti-Mouse IgG ComWin Biotech CW0102S 1: 5000 Supplementary Table S3 The ApoE genotype of Control group and AD group Control group Genotype AD group Gene type Control group #1 ɛ3/ɛ3 AD group #1 ɛ3/ɛ3 Control group #2 ɛ3/ɛ4 AD group #2 ɛ3/ɛ3 Control group #3 ɛ3/ɛ4 AD group #3 ɛ3/ɛ3 Control group #4 ɛ3/ɛ4 AD group #4 ɛ4/ɛ4 Control group #5 #N/A AD group #5 ɛ3/ɛ4 Control group #6 ɛ3/ɛ3 AD group #6 #N/A Control group #7 ɛ4/ɛ4 AD group #7 ɛ3/ɛ4 Control group #8 #N/A AD group #8 ɛ3/ɛ4 Control group #9 ɛ3/ɛ3 AD group #9 ɛ3/ɛ3 Control group #10 ɛ3/ɛ3 AD group #10 ɛ3/ɛ4 Control group #11 ɛ3/ɛ3 AD group #11 ɛ2/ɛ3 Control group #12 ɛ3/ɛ3 AD group #12 ɛ3/ɛ4 Control group #13 ɛ2/ɛ3 AD group #13 #N/A Control group #14 ɛ3/ɛ3 AD group #14 ɛ3/ɛ4 Control group #15 ɛ3/ɛ3 AD group #15 ɛ3/ɛ4 Control group #16 ɛ3/ɛ3 AD group #16 ɛ3/ɛ4 Control group #17 ɛ3/ɛ4 AD group #17 ɛ2/ɛ4 Control group #18 ɛ3/ɛ3 AD group #18 ɛ3/ɛ4 Control group #19 ɛ3/ɛ4 AD group #19 ɛ4/ɛ4 Control group #20 ɛ3/ɛ3 AD group #20 ɛ3/ɛ3 AD group #21 #N/A AD group #22 ɛ3/ɛ3 AD group #23 #N/A AD group #24 ɛ3/ɛ3 AD group #25 #N/A ..
Article Title: NCAPD2 augments the tumorigenesis and progression of human liver cancer via the PI3K-Akt-mTOR signaling pathway
Article Snippet: .. Antibody Manufacturer Cat. no. Dilution Molecular weight, kDa NCAPD2 Wuhan Sanying Biotechnology 13382-1-AP WB,1:1,000; IHC, 1:400 157 Ki67 Maxim Biotechnology RMA-0731 IHC, 1:400 Not applicable AKT Cell Signaling Technology, Inc. 4691S WB,1:1,000 60 p-AKT Cell Signaling Technology, Inc. 4060S WB,1:1,000 60 PI3K Cell Signaling Technology, Inc. 4257S WB,1:1,000 85 p-PI3K Cell Signaling Technology, Inc. 4228S WB,1:1,000 60 mTOR Cell Signaling Technology, Inc. 2983S WB,1:1,000 289 p-mTOR Cell Signaling Technology, Inc. 5536S WB,1:1,000 289 c-Myc Abcam ab32072 WB,1:1,000 57 E-cadherin Cell Signaling Technology, Inc. 3195S WB,1:1,000 135 N-cadherin Cell Signaling Technology, Inc. 13116S WB,1:1,000 140 Vimentin Cell Signaling Technology, Inc. 5741S WB,1:1000 57 GAPDH Wuhan Sanying Biotechnology 60004-1-Ig WB,1:50,000 36 mTOR, mammalian target of rapamycin; NCAPD2, non-SMC condensin I complex subunit D2; p-, phosphorylated; PI3K, phosphatidylinositol 3-kinase. ..
Concentration Assay:Article Title: PACAP–Sirtuin3 alleviates cognitive impairment through autophagy in Alzheimer’s disease
Article Snippet: .. ATG5 Forward GCTTTTGCCAAGAGTCAGCTAT Reverse AACCAATTGGATAATGCCATTTCAG Beclin1 Forward AGGAACTCACAGCTCCATTACT Reverse CTCTCCTGAGTTAGCCTCTTCC GAPDH Forward GACCCCTTCATTGACCTCAAC Reverse TTCTCCATGGTGGTGAAGA Supplementary Table S2 Antibodies used in western blot Target Supplier Catalog Number Concentration Aβ1-16 Biolegend 803001 1: 1000 Tau-5 BD Biosciences 556319 1: 1000 PACAP Invitrogen ZP006 1: 1000 Map1LC3 Cell Signaling Technology 4108s 1: 1000 Atg5 Cell Signaling Technology 2630s 1: 1000 Beclin1 Cell Signaling Technology 3738s 1: 1000 Sirt3 Cell Signaling Technology 5490s 1: 1000 PI3K Cell Signaling Technology 4249s 1: 1000 P-PI3K Cell Signaling Technology 4228s 1: 1000 AKT Cell Signaling Technology 9272s 1: 1000 P-AKT Cell Signaling Technology 4060s 1: 1000 mTOR Cell Signaling Technology 2983s 1: 1000 p-mTOR Cell Signaling Technology 2971s 1: 1000 AMPK Cell Signaling Technology 2793s 1: 1000 p-AMPK Cell Signaling Technology 2535s 1: 1000 Beta Actin Santa Cruz Biotechnology sc-47778 1: 5000 Goat Anti-Rabbit IgG ComWin Biotech CW0103S 1: 5000 Goat Anti-Mouse IgG ComWin Biotech CW0102S 1: 5000 Supplementary Table S3 The ApoE genotype of Control group and AD group Control group Genotype AD group Gene type Control group #1 ɛ3/ɛ3 AD group #1 ɛ3/ɛ3 Control group #2 ɛ3/ɛ4 AD group #2 ɛ3/ɛ3 Control group #3 ɛ3/ɛ4 AD group #3 ɛ3/ɛ3 Control group #4 ɛ3/ɛ4 AD group #4 ɛ4/ɛ4 Control group #5 #N/A AD group #5 ɛ3/ɛ4 Control group #6 ɛ3/ɛ3 AD group #6 #N/A Control group #7 ɛ4/ɛ4 AD group #7 ɛ3/ɛ4 Control group #8 #N/A AD group #8 ɛ3/ɛ4 Control group #9 ɛ3/ɛ3 AD group #9 ɛ3/ɛ3 Control group #10 ɛ3/ɛ3 AD group #10 ɛ3/ɛ4 Control group #11 ɛ3/ɛ3 AD group #11 ɛ2/ɛ3 Control group #12 ɛ3/ɛ3 AD group #12 ɛ3/ɛ4 Control group #13 ɛ2/ɛ3 AD group #13 #N/A Control group #14 ɛ3/ɛ3 AD group #14 ɛ3/ɛ4 Control group #15 ɛ3/ɛ3 AD group #15 ɛ3/ɛ4 Control group #16 ɛ3/ɛ3 AD group #16 ɛ3/ɛ4 Control group #17 ɛ3/ɛ4 AD group #17 ɛ2/ɛ4 Control group #18 ɛ3/ɛ3 AD group #18 ɛ3/ɛ4 Control group #19 ɛ3/ɛ4 AD group #19 ɛ4/ɛ4 Control group #20 ɛ3/ɛ3 AD group #20 ɛ3/ɛ3 AD group #21 #N/A AD group #22 ɛ3/ɛ3 AD group #23 #N/A AD group #24 ɛ3/ɛ3 AD group #25 #N/A ..
Control:Article Title: PACAP–Sirtuin3 alleviates cognitive impairment through autophagy in Alzheimer’s disease
Article Snippet: .. ATG5 Forward GCTTTTGCCAAGAGTCAGCTAT Reverse AACCAATTGGATAATGCCATTTCAG Beclin1 Forward AGGAACTCACAGCTCCATTACT Reverse CTCTCCTGAGTTAGCCTCTTCC GAPDH Forward GACCCCTTCATTGACCTCAAC Reverse TTCTCCATGGTGGTGAAGA Supplementary Table S2 Antibodies used in western blot Target Supplier Catalog Number Concentration Aβ1-16 Biolegend 803001 1: 1000 Tau-5 BD Biosciences 556319 1: 1000 PACAP Invitrogen ZP006 1: 1000 Map1LC3 Cell Signaling Technology 4108s 1: 1000 Atg5 Cell Signaling Technology 2630s 1: 1000 Beclin1 Cell Signaling Technology 3738s 1: 1000 Sirt3 Cell Signaling Technology 5490s 1: 1000 PI3K Cell Signaling Technology 4249s 1: 1000 P-PI3K Cell Signaling Technology 4228s 1: 1000 AKT Cell Signaling Technology 9272s 1: 1000 P-AKT Cell Signaling Technology 4060s 1: 1000 mTOR Cell Signaling Technology 2983s 1: 1000 p-mTOR Cell Signaling Technology 2971s 1: 1000 AMPK Cell Signaling Technology 2793s 1: 1000 p-AMPK Cell Signaling Technology 2535s 1: 1000 Beta Actin Santa Cruz Biotechnology sc-47778 1: 5000 Goat Anti-Rabbit IgG ComWin Biotech CW0103S 1: 5000 Goat Anti-Mouse IgG ComWin Biotech CW0102S 1: 5000 Supplementary Table S3 The ApoE genotype of Control group and AD group Control group Genotype AD group Gene type Control group #1 ɛ3/ɛ3 AD group #1 ɛ3/ɛ3 Control group #2 ɛ3/ɛ4 AD group #2 ɛ3/ɛ3 Control group #3 ɛ3/ɛ4 AD group #3 ɛ3/ɛ3 Control group #4 ɛ3/ɛ4 AD group #4 ɛ4/ɛ4 Control group #5 #N/A AD group #5 ɛ3/ɛ4 Control group #6 ɛ3/ɛ3 AD group #6 #N/A Control group #7 ɛ4/ɛ4 AD group #7 ɛ3/ɛ4 Control group #8 #N/A AD group #8 ɛ3/ɛ4 Control group #9 ɛ3/ɛ3 AD group #9 ɛ3/ɛ3 Control group #10 ɛ3/ɛ3 AD group #10 ɛ3/ɛ4 Control group #11 ɛ3/ɛ3 AD group #11 ɛ2/ɛ3 Control group #12 ɛ3/ɛ3 AD group #12 ɛ3/ɛ4 Control group #13 ɛ2/ɛ3 AD group #13 #N/A Control group #14 ɛ3/ɛ3 AD group #14 ɛ3/ɛ4 Control group #15 ɛ3/ɛ3 AD group #15 ɛ3/ɛ4 Control group #16 ɛ3/ɛ3 AD group #16 ɛ3/ɛ4 Control group #17 ɛ3/ɛ4 AD group #17 ɛ2/ɛ4 Control group #18 ɛ3/ɛ3 AD group #18 ɛ3/ɛ4 Control group #19 ɛ3/ɛ4 AD group #19 ɛ4/ɛ4 Control group #20 ɛ3/ɛ3 AD group #20 ɛ3/ɛ3 AD group #21 #N/A AD group #22 ɛ3/ɛ3 AD group #23 #N/A AD group #24 ɛ3/ɛ3 AD group #25 #N/A ..
Quantitative RT-PCR:Article Title: TrkC protects against osteoarthritis progression by maintaining articular cartilage homeostasis
Article Snippet: .. Table S1 Primers used in the qRT-PCR assay Genes Forward (5′-3′) Reverse (5′-3′) TrkA TGTCCAAGTCAGCGTCTCCT AGCACAGAGCCGTTGAACAA TrkB GCCTACACGACGAACCTCTTG GAGACAATGCCAGAAGCGAGTT TrkC GTGGCTGTTATCAGTGGAGAGG ATGTGTCTGGCTTGTGGCAAT Sox9 CGTGGACATCGGTGAACTGAG GGTGCTGCTGATGCCGTAAC Col2a1 GCTACACTCAAGTCACTGAACAACCA TCAATCCAGTAGTCTCCGCTCTTCC MMP13 GGAGCCCTGATGTTTCCCAT GTCTTCATCGCCTGGACCATA GAPDH AGGTCGGTGTGAACGGATTTG TGTAGACCATGTAGTTGAGGTCA Table S2 The detailed information of antibodies Antibodies Source Identifier TrkC Proteintech 11999-1-AP Sox9 Abcam ab185966 Clo2a1 Abcam ab34712 MMP13 Abcam ab39012 Bax Cell Signaling Technology #2772 Bcl2 Cell Signaling Technology #3498 Cleaved-Caspase3 Cell Signaling Technology #9661 Cleaved-PARP Cell Signaling Technology #9541 p-PI3K Cell Signaling Technology #4228 PI3K Cell Signaling Technology #4292 p-Akt Cell Signaling Technology #4060 Akt Cell Signaling Technology #4691 GAPDH Cell Signaling Technology #5174 Anti-rabbit IgG (H+L) Cell Signaling Technology #5151 ..
Molecular Weight:Article Title: NCAPD2 augments the tumorigenesis and progression of human liver cancer via the PI3K-Akt-mTOR signaling pathway
Article Snippet: .. Antibody Manufacturer Cat. no. Dilution Molecular weight, kDa NCAPD2 Wuhan Sanying Biotechnology 13382-1-AP WB,1:1,000; IHC, 1:400 157 Ki67 Maxim Biotechnology RMA-0731 IHC, 1:400 Not applicable AKT Cell Signaling Technology, Inc. 4691S WB,1:1,000 60 p-AKT Cell Signaling Technology, Inc. 4060S WB,1:1,000 60 PI3K Cell Signaling Technology, Inc. 4257S WB,1:1,000 85 p-PI3K Cell Signaling Technology, Inc. 4228S WB,1:1,000 60 mTOR Cell Signaling Technology, Inc. 2983S WB,1:1,000 289 p-mTOR Cell Signaling Technology, Inc. 5536S WB,1:1,000 289 c-Myc Abcam ab32072 WB,1:1,000 57 E-cadherin Cell Signaling Technology, Inc. 3195S WB,1:1,000 135 N-cadherin Cell Signaling Technology, Inc. 13116S WB,1:1,000 140 Vimentin Cell Signaling Technology, Inc. 5741S WB,1:1000 57 GAPDH Wuhan Sanying Biotechnology 60004-1-Ig WB,1:50,000 36 mTOR, mammalian target of rapamycin; NCAPD2, non-SMC condensin I complex subunit D2; p-, phosphorylated; PI3K, phosphatidylinositol 3-kinase. ..
Immunohistochemistry:Article Title: NCAPD2 augments the tumorigenesis and progression of human liver cancer via the PI3K-Akt-mTOR signaling pathway
Article Snippet: .. Antibody Manufacturer Cat. no. Dilution Molecular weight, kDa NCAPD2 Wuhan Sanying Biotechnology 13382-1-AP WB,1:1,000; IHC, 1:400 157 Ki67 Maxim Biotechnology RMA-0731 IHC, 1:400 Not applicable AKT Cell Signaling Technology, Inc. 4691S WB,1:1,000 60 p-AKT Cell Signaling Technology, Inc. 4060S WB,1:1,000 60 PI3K Cell Signaling Technology, Inc. 4257S WB,1:1,000 85 p-PI3K Cell Signaling Technology, Inc. 4228S WB,1:1,000 60 mTOR Cell Signaling Technology, Inc. 2983S WB,1:1,000 289 p-mTOR Cell Signaling Technology, Inc. 5536S WB,1:1,000 289 c-Myc Abcam ab32072 WB,1:1,000 57 E-cadherin Cell Signaling Technology, Inc. 3195S WB,1:1,000 135 N-cadherin Cell Signaling Technology, Inc. 13116S WB,1:1,000 140 Vimentin Cell Signaling Technology, Inc. 5741S WB,1:1000 57 GAPDH Wuhan Sanying Biotechnology 60004-1-Ig WB,1:50,000 36 mTOR, mammalian target of rapamycin; NCAPD2, non-SMC condensin I complex subunit D2; p-, phosphorylated; PI3K, phosphatidylinositol 3-kinase. ..
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